Video 2. High spatial resolution ICC-SM Ca²⁺ signals composed of multiple Ca^2+-firing sites.

Download video file ^{(1.9MB, mp4)}

A video showing subcellular Ca²⁺ transients in ICC-SM at high resolution imaged with a ×60 objective. Ca²⁺ signals were monitored using the genetically encoded Ca²⁺ indicator GCaMP6f. The left panel shows typical stellate-shaped ICC-SM with multiple interconnected processes. The scale bar (yellow) is 10 µm. The middle panel shows the Ca²⁺ particle (PTCL) activity, color coded in blue for raw PTCLs, and the centroids of particles are indicated in purple and green indicates Ca²⁺-firing sites. Note the multiple-site firing of Ca²⁺ transients in ICC-SM. The right panel shows initiation/firing sites accumulation map. The pattern of firing sites Ca²⁺ activity was temporally clustered as Ca²⁺ wave oscillations swept through ICC-SM networks.