Table 1. List of primary antibodies used.
Antigen retrieval protocol (30 min in 65°C water bath or 15 min in 95°C decloaking chamber) was performed for the indicated samples (e: embryos; p: pups).
| Antigen | Host | Dilution | Vendor | Catalog # | 65°C | 95°C |
|---|---|---|---|---|---|---|
| ALDH1L1 | Rabbit | 1:500 | Abcam | ab87117 | e | |
| BLBP | Rabbit | 1:200 | Millipore | ABN14 | ||
| Caspase | Rabbit | 1:400 | R and D system | AF835 | ||
| GFAP-Cy3 | Mouse | 1:500 | Sigma | C9205 | ||
| GLAST | Guinea pig | 1:200 | Millipore | AB1782 | ||
| LEF1 | Rabbit | 1:200 | Cell Signalling | 2230P | e | |
| NF1A | Rabbit | 1:500 | Active Motif | 39397 | e | |
| NF1B | Rabbit | 1:200 | Abcam | ab186738 | e | |
| PAX6 | Rabbit | 1:300 | Covance | PRB-278P | e | |
| PROX1 | Rabbit | 1:500 | BioLegend | PRB-238C | e, p | |
| REELIN | Mouse | 1:200 | Abcam | ab78540 | e | |
| SOX2 | Goat | 1:500 | Neuromics | GT15098 | e | p |
| SOX9 | Goat | 1:200 | R and D system | AF3075 | e | |
| TBR2 | Rabbit | 1:500 | Abcam | ab23345 | e, p | |
| YFP | Rat | 1:1000 | Fine chemical products | 04404–84 |