Figure 2.

SNHG14 is involved in the oxidative stress, inflammation, and apoptosis of LPS-induced HK-2 cells. (a–n) HK-2 cells were transfected with SNHG14 overexpression plasmids, and LPS-induced HK-2 cells were transfected with SNHG14 shRNAs; SNHG14 expressions were detected with qRT-PCR (a, b); oxidative stress was assessed by detecting MDA levels (c, d) and relative ROS (e, f); inflammatory cytokines including TNF-α, IL-1β, and IL-6 were measured at the transcriptional level through qRT-PCR (g, h) and in a cell supernatant using ELISA assays (i, j); cell apoptosis was analyzed using flow cytometry (k, l) and by detecting apoptosis-related biomarkers using western blot. Data were shown as mean ± SD, ^∗P < 0.05.