Figure 4.

SNHG14/miR-93 activates NF-κB and STAT3 signaling through mediating IRAK4 and IL-6R. (a) Predicted binding site of miR-93 in IRAK4 and IL-6R 3′UTR. (b, c) Luciferase assays showed that overexpressing miR-93 decreased the activity of luciferase reporter containing wild-type IRAK4 or IL-6R 3′UTR. (d) IRAK4 and IL-6R protein levels detected by western blot after HK-2 cells were transfected with miR-93 mimics or inhibitor. (e, f) HK-2 cells were transfected with SNHG14 overexpression plasmids and miR-93 mimics, and LPS-induced HK-2 cells were transfected with SNHG14 shRNAs and miR-93 inhibitor; relative proteins in IRAK4/NF-kb and IL-6R/STAT3 signaling were analyzed using western blot. Data were shown as mean ± SD, ^∗P < 0.05.