Fig. 1.

Phenotypical analysis of the neutrophils from X‐linked chronic granulomatous disease (CGD) patients having new CYBB mutations. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2) expression of patients and their mothers’ neutrophils was measured by flow cytometry, as described in Materials and methods. Solid grey curve corresponds to dihydrorhodamine (DHR)‐loaded resting neutrophils, empty black curve corresponds to DHR‐loaded neutrophils after phorbol myristate acetate (PMA) stimulation (a). For patient P12, the absence of gp91^phox (or NOX2) and p22^phox expression was shown by Western blotting using the primary specific antibodies 48, 449, respectively (b). Note that all the subunits of the NADPH oxidase complex are expressed in patient P15’s neutrophils (X91⁺CGD) by Western blotting using specific antibodies, as described in Material and methods (c).