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. 2021 Jan 1;5(2):125–142. doi: 10.7150/ntno.52168

Figure 1.

Figure 1

iTSL-DOX characterisation: (A) Doxorubicin release from iTSL at various temperatures in HEPES; (B) for 3 min at various temperatures in comparison with buffer containing 50 % (v/v) fetal bovine serum (FBS). Release is monitored by the increase of intrinsic doxorubicin fluorescence (Ex480/Em590 nm) as it leaves the self-quenched encapsulated state (n = 3; mean ± SD); (C) Liquid-phase differential scanning calorimetry of iTSL without and; (D) with encapsulated doxorubicin. Each study consisted of 3x sequential heating-cooling rounds from 25-70 °C at 1 °C/min. iTSL were in HEPES/glucose buffer and used the same as a thermal reference. Indicative onset (Ton), melting (Tm), and closure temperatures (Tcl) for each heating thermograph are: iTSL-NO-DOX: (first) Ton 41.2, Tm 43.3, Tcl 45.8, (second) Ton 41.1, Tm 43.3, Tcl 46.0; iTSL-DOX: (first) Ton 40.3, Tm 42.7, Tcl 46.0, (second) Ton 40.9, Tm 43.2, Tcl 46.0; all ± 0.2 °C. Ton/cl were calculated as the first and last temperatures at which the thermal power was 5 % of Tm.