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. 2021 Jan 1;11(6):3000–3016. doi: 10.7150/thno.47354

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Overexpression of ALKBH5 promotes heart regeneration of juvenile mice (7-day old) after MI. (A) Schematic diagram for experimental procedure. (B) Western blot analysis of ALKBH5 in in hearts from P28 AAV9-ALKBH5 mice and AAV9-control mice after injected with virus at P7. (C) m⁶A dot blot assay of mRNAs in hearts from P28 AAV9-ALKBH5 mice and AAV9-control mice after injected with virus at P7. (D, E) Infarct size of hearts from MI+AAV9-ALKBH5 and MI+AAV9-control mice by 2,3,5-triphenyltetrazolium chloride (TTC) staining. ***P < 0.001. (F, G) Cardiac function of P28 AAV9-ALKBH5 mice and AAV9-control mice after treated with MI at P7. n = 5 for SHAM and MI+AAV9-CTL mice, n = 6 for MI+AAV9-ALKBH5 mice. ***P < 0.001. (H) pH3 immunofluorescence staining in P28 SHAM, MI+AAV9-ALKBH5 mice and MI+AAV9-control hearts and quantification of pH3-positive CMs (30970 CMs in the SHAM group, 7893 CMs in the MI+AAV9-control group and 16134 CMs in the MI+AAV9-ALKBH5 group). ***P < 0.001. (I) Aurora B kinase immunofluorescence staining in P28 AAV9-ALKBH5 mice and AAV9-control hearts and quantification of pH3-positive CMs (9266 CMs in the AAV9-control group and 9549 CMs in the AAV9-ALKBH5 group). ***P < 0.001.