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. 2021 Jan 1;11(6):3000–3016. doi: 10.7150/thno.47354

Figure 6.

Figure 6

Overexpression of ALKBH5 promotes heart regeneration of adult mice (8-week old) after MI. (A) Schematic diagram for experimental procedure. (B, C) Cardiac function of AAV9-ALKBH5 mice and AAV9-control mice at day 21 after MI. n = 4 for SHAM mice, n = 6 for MI+AAV9-CTL mice and MI+AAV9-ALKBH5 mice. ***P < 0.001. (D, E) Infarct size of hearts in SHAM, MI+AAV9-ALKBH5 and MI+AAV9-control mice by 2,3,5-triphenyltetrazolium chloride (TTC) staining. **P < 0.05, ***P < 0.001. (F, G) pH3 immunofluorescence staining in MI+AAV9-ALKBH5 and MI+AAV9-control mice and quantification of pH3-positive CMs. (16725 CMs in the AAV9-control group and 17741 CMs in the AAV9-ALKBH5 group). ***P < 0.001. Scale bars, 50 µm. (H, I) Aurora B kinase immunofluorescence staining in hearts from MI+AAV9-ALKBH5 and MI+AAV9-control mice and quantification of pH3-positive CMs (7388 CMs in the AAV9-control group and 7107 CMs in the AAV9-ALKBH5 group). ***P < 0.001. Scale bar, 20 µm.