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. 2021 Jan 1;11(6):3000–3016. doi: 10.7150/thno.47354

Figure 9.

Figure 9

ALKBH5 regulates proliferation of human cardiomyocytes. (A) HiPSC-CMs were transfected with CTL-siRNA or ALKBH5-siRNAs for 48 hr. Cells were immunostained against EdU, phospho-histone H3 (pH3), Aurora B kinase and α-actinin (marks cardiomyocytes). DAPI was used for nuclear staining. n = 5 per group. ***P < 0.001. The arrows point to EdU/pH3/Aurora B kinase-positive signal. Scale bar, 50 μm. (B) HiPSC-CMs were transfected with CTL-plasmid or ALKBH5 OE plasmid for 48 hr. Cells were immunostained against EdU, phospho-histone H3 (pH3), Aurora B kinase and α-actinin (marks cardiomyocytes). DAPI was used for nuclear staining. n = 5 per group. *P < 0.05, **P < 0.01, ***P < 0.001. The arrows point to EdU/pH3/Aurora B kinase-positive signal. Scale bar, 50 μm. (C) Western blot analysis of YAP in hiPSC-CMs transfected with control or ALKBH5 OE plasmid. (D) RT-qPCR analysis of YTHDF1 hiPSC-CMs transfected with control plasmid or ALKBH5 expressing plasmid (***P < 0.001, n = 4). (E) HiPSC-CMs were transfected with CTL-siRNA or YTHDF1-siRNA for 48 hr. Cells were immunostained against phospho-histone H3 (pH3) and α-actinin (marks cardiomyocytes). DAPI was used for nuclear staining. n = 5 per group. *P < 0.05. (F) HiPSC-CMs were transfected with CTL-plasmid or YTHDF1 OE plasmid for 48 hr. Cells were immunostained against phospho-histone H3 (pH3) and α-actinin (marks cardiomyocytes). DAPI was used for nuclear staining. n = 5 per group. *P < 0.05.