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. 2021 Jan 1;11(6):2892–2916. doi: 10.7150/thno.50928

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The different nano-complex formulations decreased metastasis of tumor cells from tumor sites. (A) Spleen metastasis indicated by the yellow arrow showing hematoxylin and eosin (H&E) staining. (B) Matrix metalloproteinases (MMPs) associated with metastasis were analyzed by immunofluorescence staining of MMP9. Blue: nucleus, Green: the expression of MMP9. The data were analyzed by automatic multispectral imaging system (PerkinElmer Vectra II). Scale bar, 100 μm. For B, the quantification is shown in Figure S16. Three mice were analyzed in every group (n = 3), and one representative image per group is displayed. Data are the mean ± SEM and representative of three independent experiments.