Figure 1.

Inhibition of JMJD3 with GSKJ4 aggravates renal fibrosis following ureteral obstruction. (A) Photomicrographs illustrating Masson trichrome staining of kidney tissue (Original magnification ×200). (B) The percentage of Masson trichrome-positive tubulointerstitial area (blue) relative to the whole area was quantified. (C) The whole kidney tissue lysates from contralateral non-obstructed (Sham) and obstructed (UUO) were subject to immunoblot analysis with specific antibodies against α-SMA, fibronectin, collagen III, JMJD3, H3K27me3 or β-tubulin. Expression levels of α-SMA (D), fibronectin (E), and collagen III (F), JMJD3 (G) and H3K27me3 (H) were quantified by densitometry analysis and then normalized with β-tubulin. (I) Photomicrographs illustrating immunohistochemical staining of JMJD3 (original magnification, ×200). Scale bar= 50 µm. Data are means ± sem.*P < 0.05; **P < 0.01 versus sham controls.