Figure 2.

Inhibition of JMJD3 with GSKJ4 accelerates renal dysfunction and renal fibrosis in mice with SNx. (A) Schematic experimental design of SNx, and both blood and kidney samples were collected at 8 weeks after removal of right kidneys. (B) Serum creatinine levels. (C) Blood urea nitrogen. (D) Photomicrographs illustrating Masson trichrome staining of kidney tissue. Original magnification, ×200. (E) The percentage of Masson trichrome-positive tubulointerstitial area (blue) relative to the whole area were quantified. (F) Whole kidney tissue lysates from non-surgical (Sham) and remnant kidneys after surgery (SNx) were subject to immunoblot analysis with specific antibodies against α-SMA, fibronectin, collagen III, JMJD3, H3K27me3 or β-tubulin. Expression levels of α-SMA (G), fibronectin (H), collagen III (I), JMJD3 (J) and H3K27me3 (K) were quantified by densitometry analysis and then normalized with β-tubulin. (L) Photomicrographs illustrating immunohistochemical staining of JMJD3 (Original magnification ×200). Scale bar= 50 µm. Data are means ± sem.*P < 0.05; **P < 0.01.