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. 2021 Jan 1;11(6):2706–2721. doi: 10.7150/thno.48679

Figure 6.

Figure 6

Pharmacological and genetic inhibition of JMJD3 aggravates expression of Notch1, Notch3 and Jagged-1 in the kidney following SNx. (A) The kidney tissue lysates from non-surgical (Sham) and remnant kidneys after surgery (SNx) were subject to immunoblot analysis with specific antibodies Notch1, Notch3, Jagged-1 or β-actin. Expression levels of Notch1 (B), Notch3 (C), Jagged-1 (D), FBXW7 (E) or β-actin were quantified by densitometry analysis and then normalized with β-actin. (F) Foxd1-cre-:JMJD3fl/fl (JMJD3-WT) and Foxd1-cre+:JMJD3fl/fl (JMJD3-KO) mice were left to shamed operation or subjected to SNx and sacrificed 8 weeks after operations. The whole kidney tissue lysates were subjected to immunoblot analysis with specific antibodies against Notch1, Notch3, Jagged-1, FBXW7 or β-actin. Expression levels of Notch1 (G), Notch3 (H), Jagged-1 (I), FBXW7 (J) or β-actin were quantified by densitometry analysis and normalized with β-actin. Values are the means ± sem of 6 samples. *P < 0.05; **P < 0.01.