. 2021 Jan 12;2(1):100267. doi: 10.1016/j.xpro.2020.100267

Table 1.

Expected number of cells after each main step

Outcome	Result
Total cells post-tumor dissociation	1 to 100 million of cells depending of the tumor sample, 50%–80% viability
CD8⁺ cells post-MACS isolation	0.2 to 30 million, purity 50%–95%
Tumor cells post-MACS isolation	0.1 to 20 million, purity 80%–95%
CD8⁺CD103⁺ T_RM cells post-FACS	10,000 to 500,000 cells, purity 95%–100% (Figure 3)