Culture medium
| Final concentration | Amount | |
|---|---|---|
| RPMI 1640 | n/a | 450 mL |
| Penicillin streptomycin | 40 U/mL | 2 mL |
| 10× sodium pyruvate | 1× | 5 mL |
| Human serum | 10% | 50 mL |
| Total | n/a | 500 mL |
Store at 4°C for up to 1 month.
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