REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC Granzyme B anti-human mAb (GB12)	Thermo Fisher	Cat# MHGB05
PE-Cyanine7 NKp46 anti-mouse mAb (29A1.4)	Thermo Fisher	Cat# 25-3351-82
PerCP-Cy5.5 anti-mouse NK-1.1 mAb (PK136)	BD Biosciences	Cat# 561111
Brilliant Violet 605 anti-mouse CD45 mAb (30-F11)	BioLegend	Cat# 103139
Pacific Blue anti-mouse CD3e(500A2)	BD Pharminogen	Cat#558214
Chemicals, peptides, and recombinant proteins
Mouse BD Fc Block	BD Bioscience	Cat# 553142
Goat serum (heat-inactivated)	Sigma-Aldrich	Cat#9023
HyClone classical liquid media: RPMI 1640	Thermo Fisher	Cat# SH30027LS
Fetal bovine serum	Sigma-Aldrich	Cat# F0926
Cytofix/Cytoperm fixation and permeabilization solution	BD Bioscience	Cat# BDB554722
Murine IL-15	Peprotech	Cat#210-15
Poly(I:C) LMW	Invivogen	Cat#tlrl-picw
7-AAD	Sigma-Aldrich	Cat#A9400
Hyclone L-glutamine	Thermo Fisher	Cat# SH3003402
Hyclone HEPES solution	Thermo Fisher	Cat# SH3023701
HyClone non-essential amino acids	Thermo Fisher	Cat# SH3023801
HyClone sodium pyruvate solution	Thermo Fisher	Cat# SH3023901
HyClone penicillin streptomycin 100× solution	Thermo Fisher	Cat# SV30010
Potassium bicarbonate	Fisher Scientific	Cat#235-500
0.5 M EDTA solution	Corning	Cat#46-034-Cl
Ammonium chloride	Sigma	Cat# A9434
HyClone DPBS (calcium, magnesium)	GE	Cat# SH09928.03
2-Mercaptoethanol (2-ME)	Life Technologies	Cat# 21985-023
Critical commercial assays
CellTrace Violet Cell Proliferation Kit	Thermo Fisher	Cat# C34557
EasySep Mouse NK Cell Isolation Kit	STEMCELL Technologies	Catalog # 19855
eBioscience fixation/permeabilization Concentrate	Invitrogen	Cat# 00-5123-43
eBioscience Fixation/Permeabilization diluent	Invitrogen	Cat# 00-5223-56
Permeabilization buffer (10×)	Invitrogen	Cat#00-8333-56
Experimental models: cell lines
Mouse: RMA-S	ATCC	RRID:CVCL_2180
Experimental models: organisms/strains
Mice (8–12 weeks old) to serve as your source of NK cells (2–3 mice per group will be pooled together)	N/A	N/A
Other
Biosafety cabinet	N/A	N/A
Cell culture incubator	N/A	N/A
Dissection tools	N/A	N/A
Beckman Coulter Allegra X-15R centrifuge (or similar)	N/A	N/A
Beckman Coulter Gallios flow cytometer (or similar)	N/A	N/A
Nexcelom cellometer (or similar)	N/A	N/A
EasySep magnet	STEMCELL Technologies	Cat#18000
1 L/500 mL 0.22 μm filter	Fisher	REF# 09-761-107
70 μm cell filter	Fisher	REF#352350
100 mm × 15 mm Petri dish or similar	N/A	N/A
Insulin syringe or similar	N/A	N/A
15-mL conical tubes	N/A	N/A
T-75 cell culture flasks	N/A	N/A
96-well staining plate	Fisher	REF#3365
3 mL syringe	BD	REF#309657
5 mL polypropylene round-bottom tube	Fisher	REF#352063
96-well cell culture plate, V-bottom with lid	Fisher	REF#3894