Media and buffer

Complete RPMI (cRPMI) media
Reagent	Amount	Final concentration
RPMI 1640 medium	430 mL	–
Heat-inactivated FBS	50 mL	10%
100× Penicillin/streptomycin	5 mL	1%
Non-essential amino acids	5 mL	1%
Sodium pyruvate	5 mL	1%
L-Glutamine	5 mL	1%
Total volume	500 mL	–