| 10× ACK Lysis buffer | ||
|---|---|---|
| Reagent | Amount | Final concentration |
| Ammonium chloride | 80.24 g | 1.5 M |
| Potassium bicarbonate | 10.01 g | 0.1 M |
| 0.5 M EDTA solution | 2 mL | 1 mM |
| DPBS | to 1 L | – |
| Total volume | 1 L | 1 L |
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