Fig. 2.

Apoptotic cell death of PC KD MDA-MB-231 cells. (A) distribution of cell population in live, early apoptosis, late apoptosis and dead cell subpopulations, respectively. (B) Percentages of apoptotic cells are shown as the means ± SD from three independent experiments. ***p < 0.005. (C) Representative of Western immunoblot of procaspase 3, PARP and leaved PARP in PC KD clones 4B#3 and 4B#4, and SC cell lines at various time points. (D) Relative abundance of procaspase 3, PARP and cleaved PARP in PC KD clones. Intensity of these protein bands was normalized with that of β-actin as relative expression. The relative value of procaspase 3, PARP and cleaved PARP was relative to that of the scrambled control cell line, which was arbitrarily set to 1 at each time point. ND = Non-detectable.