NRF2 is required for FGF10-mediated hepatocellular protection in liver IRI. Mice were subjected to 90 min of partial liver warm ischemia, followed by 6 h of reperfusion. (A) Representative H&E staining, TUNEL staining, CD68 IHC staining, MPO IHC staining, and DHE staining of liver sections from NRF2 knockout mice treated with AAV-GFP/AAV-Fgf10 after IRI (magnification × 100). (B) Serum levels of ALT and AST in NRF2 knockout mice treated with AAV-GFP/AAV-Fgf10 after IRI. (C) Statistical results of panel A. (D) Levels of LPO and GSSG/GSH ratio in the livers of NRF2 knockout mice treated with AAV-GFP/AAV-Fgf10 after IRI. (E) The mRNA levels of pro-inflammatory factors (Tnf-α, Il-1β, Il-6, Ccl2, and Cxcl2) in the livers of NRF2 knockout mice treated with AAV-GFP/AAV-Fgf10 after IRI. All data are presented as mean ± SD, n = 4–6 mice/group. N.S.: non-significant.