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. 2021 Jan 13;11:1185. doi: 10.1038/s41598-020-80487-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Principal component analysis of Raman spectroscopy of (a–c) B16F10 and (d–f) melanoma phantoms: (a,d) map of PCA component corresponding to melanin; (b,e) melanin-rich area selected by Otsu thresholding of PCA component map; (c,f) brightfield image, grey frame shows the scanning area; (g) Raman spectra of the component in PCA corresponding to melanin and (h) average Raman spectra with a standard deviation of masked areas of B16F10 and melanoma phantom samples.