Figure 3.

iMSI and histological localization of dystrophin in human muscle. iMSI for dystrophin using a Gd158-conjugated primary antibody (a,e,i,m). Dystrophin immunohistochemistry (b,f,j,n) and immunofluorescence (c,g,k,o). Laminin immunofluorescence (d,h,l,p). Healthy 1 (a–d); DMD 1 (e–h); DMD 2 (i–l); DMD 3 (m–p). Laminin immunofluorescence was used for muscle fiber localization. Quantification scales denote µg kg⁻¹ gadolinium. Scale bars denote 100 µm in all images. All immunofluorescence images were taken using Axiovision 3.0 software.