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. 2021 Jan 13;11:1120. doi: 10.1038/s41598-020-80889-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Reduction of Col IV in TG mice at late age. (a) Confocal images illustrating Col IV density and its proportion in relation to the PDCLX⁺ vessels in WT (left panel) and TG mice (right panel). (b) Quantification of Col IV density and its proportion in relation to the PDCLX⁺ vessels in WT and TG mice at age of 3 months (n = 3 WT, 3 TG); age of 8 months (n = 3 WT, 3 TG); and age of 13 months (n = 3 WT, 3TG). Two-tailed student’s t-test: *p-value < 0.05. Scale bar: 50 μm. PDCLX = Podocalyxin, Col IV = Collagen IV.