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. 2021 Jan 13;11:1120. doi: 10.1038/s41598-020-80889-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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α-syn-GFP and pS129-α-syn inclusions are present in endothelial cells but not in pericytes. (a) Confocal pictures illustrating that α-syn-GFP does not co-localize with PDGFRβ⁺ pericytes (b) but shows co-localization with PDCLX⁺ blood vessels in the striatum of TG mice at age of 3, 8, and 13 months. (c) Confocal images showing pS129-α-syn does no colocalizate with CD13⁺ pericytes (d) but it colocalizes with CD31⁺ vessels in the striatum of TG mice at age of 3, 8 and 13 months. PDGFRβ = Platelet-derived growth factor receptor-beta, PDCLX = Podocalyxin, α-syn-GFP = alpha-synuclein green fluorescent protein, pS129-α-syn = phospho S129-alpha-synuclein. Scale bar: 10 µm.