Fig. 2.

Cell differentiation, development, proliferation protein and extracellular matrix protein expression by Western Blot. a, c Total protein extracted from non-treated or treated DFR extracts were analyzed by western blot with pERK, ERK2, pPKB-S473, anti-Collagen, anti-Elastin and anti-MMP-3 specific antibodies. b, d ERK and pERK, PKB and pPKB-S473 GAPDH and Collagen, Elastin, MMP-3 in the immunoprecipitates were quantified by Western analyses, as described in Methods. Bar heights are means ± SD of three independent experiments. *p < 0.05, **p < 0.01