Fig. 3.

DFR extracts effects anti-oxidant. a The antioxidant properties of DFR extract were calculated based on the DPPH radical scavenging activities. Dose-response curves were generated from which IC50 values were deduced. b NHDF cells were non-treated or treated with DFR for 24 h. Cells were harvested, lysates were analyzed by immunoblotting using anti-HO-1, Anti-NRF2 antibodies. Results are representative of three independent experiments. c GAPDH and HO-1, NRF2 in the immunoprecipitates were quantified by Western analyses, as described in Methods. Bar heights are means ±  SD of three independent experiments. d The total antioxidant capacity was plotted by measuring the absorbance at 490 nm after treating DFR extract. Uric acid and BHT (butylayed hydroxytoluene) were used as controls. *p < 0.05; **p < 0.01, versus between BHT samples; #p < 0.05, ##p < 0.01, versus between Uric acid samples; $$p < 0.01, versus between Diplectria samples