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. 2021 Jan 13;11:1224. doi: 10.1038/s41598-020-79621-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Ethambutol resistance-associated gene embC had 2 missense single nucleotide polymorphisms (SNPs) and one silent SNP (**C27181T) found in all 66 isolates in the data set as well all reference strains except H37Rv and is not considered particularly informative. All samples were wildtype for embA. Missense mutations are colored blue. Silent mutations are represented in *black color.