Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used for the quantitative RT-PCR.
| Gene | Primer | Sequence (5′–3′) |
|---|---|---|
| β-actin | Forward | TGTTACCAACTGGGACGACA |
| Reverse | GGGGTGTTGAAGGTCTCAAA | |
| COX-2 | Forward | TGAGTACCGCAAACGCTTCTC |
| Reverse | TGGACGAGGTTTTCCACCAG | |
| TNF-α | Forward | TAGCCAGGAGGGAGAACAGA |
| Reverse | TTTTCTGGAGGGAGATGTGG | |
| IL-6 | Forward | CTGGAGCCCACCAAGAACGA |
| Reverse | GCCTCCGACTTGTGAAGTGGT | |
| IL-1β | Forward | ATGCCACCTTTTGACAGTGATG |
| Reverse | GTTGATGTGCTGCTGCGAGATT | |
| VEGFA | Forward | TGAAGTGATCAAGTTCATGGACGT |
| Reverse | TCACCGCCTTGGCTTGTC |
