Table 1.
Strains used in the study.
| Abbreviation | Genotype | Description | Reference |
|---|---|---|---|
| Ss OAg− | S. sonnei-pSS tolR::kan | Hyperblebbing S. sonnei-pSS. LPS OAg-deficient rough mutant with hexa-acylated lipid A | 15 |
| Ss ΔmsbB OAg− | S. sonnei-pSS tolR::kan msbB1::cat | Hyperblebbing S. sonnei-pSS. LPS OAg-deficient rough mutant with penta-acylated (-myristoyl chain) lipid A | 12 |
| Ss ΔhtrB OAg− | S. sonnei -pSS tolR::kan htrB::cat | Hyperblebbing S. sonnei-pSS. LPS OAg-deficient rough mutant with penta-acylated (-lauroyl chain) lipid A | 12 |
| Ss OAg+ | S. sonnei virG::nadAB tolR::kan | Hyperblebbing S. sonnei. pSS stabilized, OAg positive with hexa-acylated lipid A | 14 |
| Ss ΔmsbB OAg+ | S. sonnei tolR::kan virG::nadAB msbB1::cat msbB2::ery | Hyperblebbing S. sonnei. pSS stabilized, OAg-positive with penta-acylated (-myristoyl chain) lipid A | This study |
| Ss ΔhtrB OAg+ | S. sonnei virG::nadAB tolR::kan htrB::cat | Hyperblebbing S. sonnei. pSS stabilized, OAg-positive with penta-acylated (-lauroyl chain) lipid A | 14 |
| Ss WT OAg+ | S. sonnei virG::cat | Growth in the presence of chloramphenicol selects for the presence of pSS and thus avoids loss of the OAg cluster in vitro | 20 |
| Ss WT OAg− | S. sonnei wbg::cat | LPS OAg-deficient rough mutant. Knock-out of the OAg biosynthesis cluster (genes wzz to wbgZ) | 20 |
The table summarizes all the bacterial strains used in this study.