Table 2.
Analytical characterization of the antigens.
| GMMA | Lipid A type (MALDI-TOF) | Radius (nm) (HPLC-SEC/MALS) | OAg/protein w/w ratio (HPAEC-PAD/micro BCA) |
|---|---|---|---|
| Ss OAg+ | Hexa | 35.0 | 0.25 |
| Ss ΔhtrB OAg+ | Penta | 47.0 | 0.03 |
| Ss ΔmsbB OAg+ | Penta | 42.0 | 0.22 |
| Ss OAg− | Hexa | 41.5 | No OAg detected |
| Ss ΔhtrB OAg− | Penta | 29.3 | No OAg detected |
| Ss ΔmsbB OAg− | Penta | 33.7 | No OAg detected |
The table summarizes GMMA characterization in terms of lipid A structure (MALDI-TOF), size (HPLC-SEC/MALS) and OAg to protein (w/w) ratio (HPAEC-PAD/micro BCA).