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. 2021 Jan 13;11:1211. doi: 10.1038/s41598-020-79879-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The phylogenetic status of three manatee-associated nematodes among closely-related species. The combined Bayesian tree was inferred from near full length of SSU and D2-D3 LSU under GTR + G + I model applied for both loci. The parameters are as follows: lnL = − 24,768.25718; freqA = 0.25; freqC = 0.21; freqG = 0.27; freqT = 0.27; R(a) = 1.00; R(b) = 2.88; R(c) = 2.38; R(d) = 0.88; R(e) = 4.25; R(f) = 1.00; Pinva = 0.38; Shape = 0.57) for SSU, and lnL = − 27,032.96896; freqA = 0.21; freqC = 0.22; freqG = 0.32; freqT = 0.25; R(a) = 0.43; R(b) = 1.50; R(c) = 0.79; R(d) = 0.36; R(e) = 3.07; R(f) = 1.00; Pinva = 0.20; Shape = 1.03). Posterior probability values exceeding 50% are given on appropriate clades. The full phylogenetic tree is provided in Supplementary Fig. S1.