Table 1.
Skin sampling results and relative abundance of nematodes on the Florida manatee from Crystal River, Florida during the 2018 health assessments.
| USGS manatee ID | Sample plate | Cutidiplogaster manati | LT | ST | Visible skin remnants |
|---|---|---|---|---|---|
| CCR18-19 | Sample A | +++ | +++ | +++ | P |
| Sample B | +++ | +++ | +++ | P | |
| Sample C | 0 | +++ | 0 | P | |
| Sample D | +++ | +++ | +++ | P | |
| Sample E | 0 | +++ | +++ | P | |
| Sample F | +++ | +++ | 0 | P | |
| CCR18-20 | Sample A-1 | 0 | +++ | 0 | A |
| Sample B-1 | +++ | +++ | +++ | A | |
| Sample C-1 | 0 | +++ | 0 | A | |
| Sample D-1 | 0 | +++ | +++ | A | |
| Resample A-2 | 0 | +++ | 0 | P | |
| Resample B-2 | 0 | +++ | 0 | P | |
| Resample C-2 | 0 | +++ | 0 | P | |
| Resample D-2 | +++ | +++ | 0 | P | |
| CCR18-21 | Sample A | 0 | 0 | 0 | A |
| Sample B | 0 | + | 0 | P | |
| Sample C | 0 | ++ | ++ | A | |
| Sample D | ++ | ++ | ++ | A | |
| Sample E | ++ | ++ | ++ | P | |
| Sample F | ++ | ++ | ++ | P | |
| CCR18-22 | Sample A | 0 | 0 | 0 | A |
| Sample B | + | + | 0 | A | |
| Sample C | 0 | ++ | ++ | P | |
| Sample D | 0 | ++ | ++ | A | |
| Sample E | ++ | ++ | ++ | P | |
| Sample F | ++ | ++ | ++ | A | |
| CCR18-23 | Sample A | +++ | +++ | +++ | A |
| Sample B | +++ | +++ | +++ | A | |
| Sample C | 0 | +++ | +++ | A | |
| Sample D | 0 | +++ | +++ | A | |
| Sample E | +++ | +++ | +++ | A | |
| CCR18-24 | Sample A | +++ | +++ | +++ | A |
| Sample B | +++ | +++ | +++ | A | |
| Sample C | +++ | +++ | +++ | A | |
| Sample D | +++ | +++ | +++ | A | |
| Sample E | +++ | +++ | +++ | A | |
| Sample F | +++ | +++ | +++ | A | |
| CCR18-25 | Sample A | +++ | +++ | +++ | P |
| Sample B | +++ | +++ | +++ | P | |
| Sample C | +++ | +++ | +++ | P | |
| Sample D | +++ | 0 | +++ | P | |
| Sample E | +++ | +++ | +++ | P | |
| Sample F | +++ | +++ | +++ | P | |
| Sample G | +++ | +++ | +++ | P | |
| Sample H | +++ | +++ | +++ | P | |
| Sample I | +++ | +++ | +++ | P |
Estimated measurement of abundance was recorded for each morphospecies observed on each sample plate. This was categorized and recorded as 0 = absent, + = 1–15 nematodes visible,++ = 15–99 nematodes visible, and +++ = 100 or more nematodes visible. Visible skin remnants were categorized as A = absent, or P = present. On manatee CCR18-20, sampling first used minimal pressure (samples A-1 to D-1) and then each location was resampled with increased pressure (samples A-2 to D-2). For all other sampled manatees, each different sample plate represents a pseudoreplicate sample taken from a different arbitrary location near the mid-tail dorsal surface. Samples labelled under a specific manatee ID with a different letter represent a different 5 × 5 cm mid-dorsal tail sampling on the same manatee.