Table 2.

Skin sampling results and relative abundance of nematodes on the Florida manatee from Crystal River, Florida during the 2019 health assessments.

USGS manatee ID	Sample plate	Cutidiplogaster manati	LT	ST	Visible skin remnants
CCR19-01	Sample A	+++	+++	+++	A
	resample A-1	+++	0	+++	P
	resample A-2	+++	+++	+++	P
	resample A-3	+++	+++	+++	P
CCR19-02	Sample A	+++	+++	+++	P
	resample A-1	+++	+++	+++	P
	resample A-2	+++	+++	+++	P
	resample A-3	++	++	++	P
	Sample B	+++	+++	+++	P
	Sample C	+++	+++	+++	P
	Sample D	+++	+++	+++	P
CCR19-03	Sample A	0	0	0	P
	Sample B	++	++	0	P
	Sample C	0	+	0	P
	Sample D	0	0	0	P
CCR19-04	Sample A	0	0	+	A
	resample A-1	++	++	++	P
	resample A-2	++	++	++	P
	resample A-3	++	++	++	P
CCR19-05	Sample A	0	++	++	P
	resample A-1	0	++	++	P
	resample A-2	++	++	++	P
	resample A-3	++	++	++	P
CCR19-06	Sample A	++	++	0	A
	resample A-1	0	++	0	P
	resample A-2	++	0	0	P
	resample A-3	++	++	++	P
CCR19-07	Sample A	0	+	+	P
	resample A-1	+++	+++	+++	P
	resample A-2	+++	+++	+++	P
	resample A-3	+++	+++	+++	P

Estimated measurement of abundance was recorded for each morphospecies observed on each sample plate and categorized as 0 = absent, +  = 1–15 nematodes visible,++ = 15–99 nematodes visible, and +++  = 100 or more nematodes visible. Visible skin remnants were categorized as A = absent, or P = present. All manatees except CCR19-03 were sequentially sampled using increasing pressure. Sequential sampling first used minimal pressure (sample A) and then each location was resampled with increased pressure (resamples A-1 to A-3). On manatee CCR19-03, each different sample represents a pseudoreplicate taken from a different arbitrary location near the mid-tail dorsal surface. On manatee CCR19-02, three additional pseudoreplicate sample were also taken from different arbitrary location near the mid-tail dorsal surface.