Figure 4.

Increasing neuronal glucose uptake attenuates declines in physical performance in aged flies and has an additive effect with dietary restriction (DR) in extending life span

(A) Neuronal expression of hGlut3 ameliorated the age-associated neuronal dysfunction. Flies were tapped to the bottom of the vial, and the distance that they then climbed in 30 s was measured (mean ± SE, n = 12–39; ∗p < 0.05, ∗∗∗p < 0.001; Student's t-test). Flies carrying the driver alone were used as controls.

(B–D) Neuronal expression of hGlut3 and DR synergistically extended life span. Flies were raised on regular cornmeal food and maintained on regular cornmeal food (B) or two types of DR diet (C and D) (C: food without cornmeal and containing 10% (w/v) of yeast and glucose (10%), D: food without cornmeal and containing 1% (w/v) of yeast and glucose (1%) after eclosion. Error bars represent 95% confidence intervals. Data are expressed as mean ± SE, n = 131-601, ∗∗∗; p < 0.001, ∗∗∗∗; p < 0.0001, log-rank test.