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. 2021 Jan 13;11:1057. doi: 10.1038/s41598-020-80379-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Siberian sturgeon’ small intestine (SI), pyloric caeca (PC) and spiral valve (SV) histology at t₁ and histological indexes (mucosal folds length, supranuclear vacuoles abundance and goblet cells number) calculated in these gut tracts. (a–d) small intestine; (e–h) pyloric caecum; (i–l) spiral valve. Histology scale bars: (a,c,e,g) 100 μm; (b,d,f,h,j,l) 50 μm; (i,k) 200 μm. For histological indexes (m), values are showed as mean ± SD (n = 15). Scores: supranuclear vacuoles + scattered, ++ abundant; goblet cells + 0 to 4 per villus, ++ more than 4 per villus. Significant differences, calculated within the same sampling time, between Hi0 and Hi50 are indicated as follows: ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001. Sturgeon fed diets including 0 and 50 of BSF meal (Hi0 and Hi50, respectively).