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. 2021 Jan 13;11:1057. doi: 10.1038/s41598-020-80379-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Spectral analysis of liver and small intestine samples of Siberian sturgeon collected at t₁. (a) Microphotographs of liver samples and topographical distribution of: lipids (LIP maps), fatty acids (FA maps), unsaturated alkyl chains (CH maps), proteins (PRT maps), and glycogen (GLY maps) (map size 164 × 164 µm²). (b) Statistical analysis of liver biochemical composition: LIP/TBM (relative amount of total lipids), FA/TBM (relative amount of fatty acids), CH/TBM (degree of unsaturation in lipid alkyl chains), PRT/TBM (relative amount of total proteins) and GLY/TBM (relative amounts of glycogen). (c) Microphotographs of small intestine samples and topographical distribution of: lipids (LIP maps), proteins (PRT maps) and carbohydrates (CARBO maps) (map size 328 × 164 µm²). (d) Statistical analysis of small intestine biochemical composition: LIP/TBM (relative amount of total lipids), PRT/TBM (relative amount of total proteins) and CARBO/TBM (relative amount of carbohydrates). Significant differences between Hi0 and Hi50 are indicated as follows: ns non-significant; *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001. Data are reported as mean ± SD (n = 6). Sturgeons fed diets including 0 and 50 of BSF meal (Hi0 and Hi50, respectively).