Table 1.
Fatty acid composition (as % of total FA) of experimental diets and Siberian sturgeon at t0 and t1.
| Diets | Sturgeon | |||||
|---|---|---|---|---|---|---|
| Hi0 | Hi50 | t0 | t1 | |||
| Hi0 | Hi50 | Hi0 | Hi50 | |||
| 10:0 | 0.020 ± 0.001 | 0.570 ± 0.010**** | 0.005 ± 0.001 | 0.004 ± 0.001 | 0.003 ± 0.001 | 0.004 ± 0.001 |
| 12:0 | 0.18 ± 0.01 | 8.1 ± 0.7**** | 0.15 ± 0.04 | 0.12 ± 0.03 | 0.10 ± 0.03 | 2.35 ± 0.60**** |
| 13:0 | 0.06 ± 0.001 | n.d | 0.032 ± 0.001 | 0.029 ± 0.001 | 0.030 ± 0.002 | 0.044 ± 0.005 |
| 14:0 | 5.67 ± 0.06 | 4.6 ± 0.3** | 2.80 ± 0.09 | 2.67 ± 0.04** | 3.81 ± 0.12 | 3.73 ± 0.21ns |
| 14:1n5 | 0.53 ± 0.01 | n.d | 0.34 ± 0.01 | 0.32 ± 0.01 | 0.41 ± 0.01 | 0.82 ± 0.07**** |
| 15:0 | 0.61 ± 0.01 | 0.58 ± 0.03 ns | 0.61 ± 0.01 | 0.59 ± 0.01*** | 0.60 ± 0.01 | 0.57 ± 0.01**** |
| 15:1n5 | n.d | n.d | n.d | n.d | n.d | n.d |
| 16:0 | 14.5 ± 0.3 | 17.1 ± 0.6** | 13.9 ± 0.1 | 13.7 ± 0.1*** | 14.7 ± 0.2 | 12.3 ± 0.1**** |
| 16:1n9 | 0.36 ± 0.01 | 6.3 ± 0.3**** | 0.49 ± 0.01 | 0.48 ± 0.01 | 0.49 ± 0.01 | 0.74 ± 0.06** |
| 16:1n7 | 6.25 ± 0.04 | n.d | 4.37 ± 0.01 | 4.29 ± 0.01**** | 5.81 ± 0.11 | 5.60 ± 0.42ns |
| 16:2n7 | 0.28 ± 0.01 | n.d | 0.30 ± 0.02 | 0.28 ± 0.01 | 0.13 ± 0.02 | 0.21 ± 0.01 |
| 17:0 | 0.53 ± 0.09 | n.d | 0.48 ± 0.09 | 0.55 ± 0.08 | 0.50 ± 0.01 | 0.41 ± 0.06 |
| 17:1n7 | n.d | n.d | n.d | n.d | n.d | n.d |
| 18:0 | 3.87 ± 0.01 | 13.1 ± 1.8*** | 3.21 ± 0.02 | 3.00 ± 0.02**** | 2.95 ± 0.39 | 3.44 ± 0.16** |
| 18:1n9 | 9.94 ± 0.06 | 2.30 ± 0.20**** | 17.7 ± 0.1 | 17.6 ± 0.1* | 16.5 ± 0.3 | 17.1 ± 0.2*** |
| 18:1n7 | 2.26 ± 0.01 | n.d | 2.66 ± 0.01 | 2.66 ± 0.02ns | 2.91 ± 0.04 | 2.61 ± 0.17**** |
| 18:2n6 | 9.06 ± 0.03 | 27.4 ± 2.3*** | 12.9 ± 0.1 | 12.9 ± 0.1ns | 12.5 ± 0.3 | 17.5 ± 1.3**** |
| 18:3n6 | n.d | n.d | n.d | n.d | n.d | n.d |
| 18:3n3 | 1.91 ± 0.06 | 3.4 ± 0.3** | 2.51 ± 0.01 | 2.51 ± 0.01ns | 2.03 ± 0.12 | 1.86 ± 0.06** |
| 20:0 | 0.36 ± 0.01 | n.d | 0.24 ± 0.01 | 0.23 ± 0.01 | 0.19 ± 0.01 | 0.12 ± 0.02 |
| 20:1n9 | 1.83 ± 0.03 | n.d | 3.17 ± 0.04 | 3.19 ± 0.01ns | 2.25 ± 0.12 | 1.80 ± 0.27*** |
| 20:2n6 | 0.33 ± 0.03 | n.d | 1.26 ± 0.05 | 1.24 ± 0.03ns | 1.10 ± 0.02 | 1.93 ± 0.07**** |
| 20:3n6 | 0.26 ± 0.02 | n.d | 0.57 ± 0.08 | 0.53 ± 0.02 ns | 0.53 ± 0.01 | 1.26 ± 0.07**** |
| 21:0 | n.d | n.d | n.d | n.d | n.d | n.d |
| 20:4n6 | 1.23 ± 0.01 | n.d | 1.48 ± 0.02 | 1.49 ± 0.02ns | 1.27 ± 0.04 | 2.27 ± 0.16**** |
| 20:3n3 | 0.17 ± 0.01 | n.d | 0.43 ± 0.01 | 0.43 ± 0.01 | 0.30 ± 0.02 | 0.30 ± 0.04 |
| 20:5n3 | 17.3 ± 0.2 | 4.8 ± 0.4**** | 6.15 ± 0.04 | 6.26 ± 0.03**** | 9.29 ± 0.18 | 4.51 ± 0.05**** |
| 22:0 | 0.24 ± 0.01 | n.d | 0.081 ± 0.001 | 0.077 ± 0.003 | 0.063 ± 0.003 | 0.039 ± 0.009 |
| 22:1n9 | 1.53 ± 0.03 | n.d | 1.19 ± 0.05 | 1.19 ± 0.03ns | 0.62 ± 0.06 | 0.25 ± 0.14**** |
| 23:0 | n.d | n.d | n.d | n.d | n.d | n.d |
| 24:0 | n.d | n.d | n.d | n.d | n.d | n.d |
| 22:6n3 | 20.7 ± 0.3 | 11.7 ± 1.5*** | 22.9 ± 0.1 | 23.6 ± 0.2**** | 21.0 ± 0.3 | 18.2 ± 1.7*** |
| 24:1n9 | n.d | n.d | n.d | n.d | n.d | n.d |
| DHA/EPA | 1.19 ± 0.03 | 2.5 ± 0.5* | 3.7 ± 0.1 | 3.8 ± 0.1* | 2.26 ± 0.06 | 4.0 ± 0.3**** |
Experimental diets included 0 and 50% of BSF meal respect to FM (Hi0 and Hi50). Siberian sturgeon fed diets including 0 and 50% of BSF meal (Hi0 and Hi50). Significant differences between Hi0 and Hi50 within rows and separately from diets, t0 and t1 are indicated as follows: ns non-significant; *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001. Values are shown as mean ± SD (n = 3 for experimental diets; n = 9 for sturgeon at both t0 and t1). Statistical analysis was performed only for FAs > 0.5% (FA with a percentage < 0.5% were excluded from any statistical analyses because their concentrations were close to the limit of detection).