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. 2021 Jan 13;11:877. doi: 10.1038/s41598-020-80332-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Course of disease for four dogs with lymphoma that were treated using chemotherapy (dog 1: 18182, dog 2: 18710, dog 3: 18680, dog 4: 18744) (drawing produced by R studio software Version 1.1.463; Vienna, Austria⁶⁰). For each dog, the figures above represent the tumor burden with lymph node size (sum of longest diameter of at least 2 lymph nodes; in mm). The figures below represent the ctDNA measurement (% of total cfDNA in plasma) during the disease. For each case, a Spearman correlation analysis was performed to assess the correlation between the tumor burden and ctDNA quantity (coefficient and p value). CR complete response, PR partial response, SD stable disease, PD progressive disease.