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. 2021 Jan 13;7:10. doi: 10.1038/s41420-020-00366-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Determination of inhibitory potential of small molecules against NAD⁺-glycohydrolase activity of TNT using NADH fluorescence assay. 10 µM of small-molecule inhibitors was incubated with 75 nM of TNT followed by addition of 100 µM NAD⁺ to initiate the reaction. The remaining NAD⁺ levels were then measured. NAD⁺ level of control reaction without TNT was considered as 100%. Percent decrease in NAD⁺ content represents NAD⁺-glycohydrolase activity. b Inhibition of NAD⁺-glycohydrolase activity of TNT by selected molecules 8, 9, and 10 using enzyme-coupling assay. Recombinant TNT (30 nM) was incubated with 1 µM of inhibitor followed by addition of 5 µM NAD⁺. NAD⁺-glycohydrolase activity of TNT was measured using enzyme coupling assay. The P values were calculated using unpaired t-test with Welch’s correction. *P < 0.05, **P < 0.01, ***P < 0.001. Statistical significance was determined considering TNT treated sample as the control against which other groups were compared. c 3D surface model of TNT-8, TNT-9, and TNT-10 complex. Ligplot analysis of these interactions and Hydrogen-bonding analysis of TNT-8, TNT-9, and TNT-10 complex showing minimum binding energy of −8.92 kcal/mol, −8.43 kcal/mol, and −7.33 kcal/mol, respectively.