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. 2021 Jan 13;11:948. doi: 10.1038/s41598-020-80277-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of ApTPS and ApTRE RNAi treatments on the expression of the gens involved in the chitin biosynthesis. (A) in red morphs at 24 h, (B) in green morphs at 24 h, (C) in red morphs at 48 h, (D) in green morphs at 48 h. CK, the normal diet; dsGFP, the GFP-dsRNA treatment; dsTPS, the TPS-dsRNA treatment; dsTRE, the TRE-dsRNA treatment; ApTPS, trehalose-6-phosphate synthase gene; ApTRE, trehalase gene; ApHK, Hexokinase gene; ApGPI, glucose-6-phosphate isomerase gene; ApGFAT, glutamine-fructose-6-phosphate aminotansferasr gene; ApGPN, glucosamine-6-phosphate-N-acetytransferase gene; ApUAP, UDP-N-acetyglucosamine pyrophosphorylase gene; Apchs-2, chitin synthase gene 2; ApGP, glycogen phosphorylase gene. Each bar represents the Means ± SEM from three biological replicates and three technical replicates. The data were analyzed using one-way analysis of variance (ANOVA), followed by the Tukey–Kramer test. The mRNA expression level in the normal artificial diet group was designated as the reference control for the comparisons. The different letters above the error bars indicate significant differences (P < 0.05).