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. 2021 Jan 13;11:948. doi: 10.1038/s41598-020-80277-2

Figure 5.

Figure 5

Effects of RNAi of ApTPS and ApTRE on relative expression of the chitinase and chitinase-like genes. (A) In red morphs at 24 h, (B) in green morphs at 24 h, (C) in red morphs at 48 h, (D) in green morphs at 48 h. CK, the normal diet; dsGFP, the GFP-dsRNA treatment; dsTPS, the TPS-dsRNA treatment; dsTRE, the TRE-dsRNA treatment; ApCht1, 2, 3, 5, 6, 7, 8, 10; ApIDGF, Imaginal disc growth factor gene; ApENGase, Endo-β-N-acetylgucosaminidase gene. Each bar represents the Means ± SEM from three biological replicates and three technical replicates. The data were analyzed using one-way analysis of variance (ANOVA), followed by the Tukey–Kramer test. The mRNA expression level in the normal artificial diet group was designated as the reference control for the comparisons. The different letters above the error bars indicate significant differences (P < 0.05).