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. 2021 Jan 13;11:874. doi: 10.1038/s41598-020-79536-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Mito-FF on the apoptotic cell death. (A) Quantification of flow cytometric analysis of sorafenib-sensitive/resistant Huh7 cells after AnnexinV/PI staining following sorafenib treatment. The proportion of apoptosis cells (%) were expressed as the total percentage of Annexin V-positive/PI-negative cells. (B) Quantification of flow cytometric analysis of sorafenib-sensitive/resistant Huh7 cells after AnnexinV/PI staining following Mito-FF treatment. (C) TUNEL assay to determine apoptosis of sorafenib-sensitive [left] /resistant [right] Huh7 cells by sorafenib, respectively. Compared to Huh7-S cells, fewer TUNEL-positive cells were found in Huh7-R cells after treatment with 10 μM sorafenib. (D) TUNEL assay to determine apoptosis of sorafenib-sensitive [left] /resistant [right] Huh7 cells by Mito-FF, respectively. Similar number of TUNEL-positive cells were found in Huh7-R cells as well as Huh7-S cells after treatment with 25 μM Mito-FF. Values are presented as mean ± standard deviation of three independent experiments. *P < 0.05. Abbreviations: Huh7-S cell; sorafenib-sensitive Huh7 cell; Huh7-R cell; sorafenib-resistant Huh7 cell; Mito-FF; mitochondria-accumulating phenylalanine dipeptide with triphenyl phosphonium.