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. 2021 Jan 13;11:874. doi: 10.1038/s41598-020-79536-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Mito-FF on the expression of antioxidant proteins. (A) (Top) Western blot analysis showing the expression of antioxidant proteins of sorafenib-sensitive [Left] /resistant [Right] Huh7 cells after sorafenib treatment. (Bottom) Relative densities of the markers in each group. (B) (Top) Western blot analysis showing the expression of antioxidant proteins of sorafenib-sensitive [Left] /resistant [Right] Huh7 cells after Mito-FF treatment. (Bottom) Relative densities of the markers in each group. Relative densities of individual markers had been quantified using Image J software (http://imagej.nih.gov/ij/download.html) and then were normalized to that of β-actin in each group. Values are presented as mean ± standard deviation of three independent experiments. *P < 0.05. Abbreviations: GPx, glutathione peroxidase; Huh7-S cell; sorafenib-sensitive Huh7 cell; Huh7-R cell; sorafenib-resistant Huh7 cell; Mito-FF; mitochondria-accumulating phenylalanine dipeptide with triphenyl phosphonium; SOD, superoxide dismutase.