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. 2021 Jan 13;11:984. doi: 10.1038/s41598-020-80098-3

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Persistence of SARS-CoV-2 on cotton. Ten microliters of SARS-CoV-2 (titre = 7.88 LogTCID₅₀/mL) prepared in a tripartite soil load were inoculated onto gamma-irradiated coupons of cotton fabric and sampled at predetermined intervals over a 3-day time-course. TCID₅₀ values (solid lines) represent mean ± SD of viable virus recovered from three biological replicates following end-point titration in Vero E6 cells. Genome equivalents (dashed black lines) are based on nucleoprotein-specific qRT-PCR results obtained from pooled eluates of three biological replicates at indicated sampling points. Red dashed lines indicate limits of quantification for the TCID₅₀ assay. Asterisk indicates detection of viable virus in safety test of a single biological replicate.