Figure 6.

Vector analysis reveals oligoclonal integration profile with no detectable insertions into known high-risk proto oncogenes

The following BM samples were chosen for integration analysis by the INSPIIRED platform; #059, 086, 0242, 0400, 0410, 0432, 0434, 0493, and 0494. Two Mock BM samples were used as negative controls (#0402 and 0495), these did not generate any valid integration sites. (A) LV samples were compared to matched random controls (part of the INSPIIRED pipeline) in terms of percentage (%) of insertions inside of genes. Mann-Whitney U-test ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001; ∗∗∗∗p ≤ 0.0001. Error bars represent mean + SD. (B) The ten integrations with the highest sequence contribution in each analyzed sample are depicted. The gene symbol closest to the integration site are shown. According to the INSPIIRED pipeline, an asterisk (∗) behind the gene symbol indicates that the integration was within a transcription unit and a tilde (˜) that the insertion was within 50 kb of a cancer-related gene.