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. 2020 Dec 10;20:298–311. doi: 10.1016/j.omtm.2020.12.001

Figure 2.

Figure 2

dSaCas9-mediated recruitment of epigenetic repressors to the DUX4 promoter or exon 1 represses DUX4-fl and DUX4-FL targets in FSHD myocytes

(A–D) FSHD myocytes were transduced with dSaCas9 fused to either: (A) the SUV39H1 pre-SET, SET, and post-SET domains (SET); (B) the MeCP2 TRD; (C) HP1γ; or (D) HP1α, with or without sgRNAs targeting DUX4 (#1–6) or non-targeting sgRNAs (NT). Expression levels of DUX4-fl and DUX4-FL target genes TRIM43 and MBD3L2 were assessed by qRT-PCR. Data are plotted as the mean + SD value of at least four independent experiments, with relative mRNA expression for cells expressing each dCas9-epigenetic regulator alone set to 1. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 are from comparing to NT.