Figure 4.

Targeting dSaCas9-repressors to DUX4 has minimal effects on global gene expression in FSHD myocytes

(A–E) FSHD myocytes were transduced with: (A) dSaCas9-KRAB + sgRNA #6, (B) dSaCas9-HP1α + sgRNA #2, (C) dSaCas9-HP1γ + sgRNA #5, (D) dSaCas9-SET + sgRNA #1, or (E) dSaCas9-TRD + sgRNA #6. For each treatment, five independent experiments were analyzed by RNA-seq using the Illumina HiSeq 2 × 100 bp platform. Adjusted volcano scatterplots show the global transcriptional changes between each treatment versus mock-infected cells. Each data point represents a gene. Upregulated genes (p < 0.05 and a log2 fold change > 1) are indicated by red dots. Downregulated genes (p < 0.05 and a log2 fold change < −1) are indicated by blue dots. Unique differentially expressed genes (summarized in F) are indicated by yellow dots.