METTL3 regulation of ZBTB4 is involved in the EMT and malignancy of T-HBE cells
T-HBE cells were transfected with METTL3 siRNA and/or co-transfected with a ZBTB4 siRNA. (A) Western blots were performed, and (B) relative protein levels (mean ± SD, n = 3) of METTL3, ZBTB4, EZH2, E-cadherin, N-cadherin, vimentin, and H3K27me3 were determined. (C) Colony-formation assays and Transwell assays (scale bars, 100 μm) were performed, and (D) relative colony numbers and (E) relative levels of cell invasion and migration were determined (mean ± SD, n = 3). ∗p < 0.05, different from T-HBE cells transfected with METTL3 siRNA alone.