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. 2021 Jan 13;11:975. doi: 10.1038/s41598-020-79597-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Omega-7 on the cultured keratinocyte colony-forming rate. (A) Effects of 0.025% omega-7 on cultured keratinocyte colony-forming rate (proliferation) at 72 and 96 h. (B) Effects of 0.08% of Omega-7 on cultured keratinocyte colony-forming rate at 48 h. Omega-7 with dose of 0.08% negatively affected the cells. Keratinocyte colony counting assay was performed using 24,000 cells/cm² sheep primary keratinocytes in passage 2. Cells were treated with Omega-7 at time 0 h. The colonies were counted in 12 dishes (6 treated and 6 controls). Data are expressed as mean ± SEM. Scale bar in micrographs: 200 μm. Open circles indicate cells treated with Omega-7 and closed circles represent control cells. Photographs taken and compiled, by authors, in Adobe Photoshop CC 2020 (https://www.adobe.com/jp/products/photoshop.html) without changing the content of images themselves.