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. 2021 Jan 13;11:975. doi: 10.1038/s41598-020-79597-0

Figure 7.

Figure 7

Keratinocyte growth factor (KGF), nitrotyrosine, and telomerase activity in grafted burn wounds at 14 days. (A) KGF were measured in four sheep (4 treated and 4 control sites). The level of KGF in skin tissue was significantly higher in the treatment group compared to the control group (0.21 ± 0.07 vs. 0.31 ± 0.07 relative unit). Full-length blots/gels are presented in Supplementary Fig. 1. (B) Nitrotyrosine were measured in eight sheep (8 treated and 8 control sites). The level of nitrotyrosine in skin tissue was significantly reduced in the treatment group compared to the control group (0.36 ± 0.05 vs. 0.61 ± 0.11 relative unit). Full-length blots/gels are presented in Supplementary Fig. 2. (C) Telomerase activity were measured in five sheep (6 Treated and 6 control sites). Telomerase activity measured by Telomeric Repeat Amplification Protocol (TRAP) and Gel-Based Telomerase Detection at 14 days after skin graft. The telomerase activity was significantly higher in Omega-7 grafted burn sites than that of control sites (47.1 ± 9.2 vs. 9.0 ± 0.9). The pictures show the telomerase activity of non-heat-treated samples in each group. Full-length blots/gels are presented in Supplementary Fig. 3. S-IC: signal from the internal standard in non-heat-treated samples. Data is expressed as mean ± SEM. Photographs taken and compiled, by authors, in Adobe Photoshop CC 2020 (https://www.adobe.com/jp/products/photoshop.html) without changing the content of images themselves.